Figure 7

PP2A shuttles to the plasma membrane and nuclear subcellular fractions in response to Wnt3a. Cells expressing Rfz1 receptor were harvested at indicated time point after Wnt3a stimulation. Cells were fractionated to the plasma membrane (PM), cytoplasm (CY) and nuclei (NU), as described in the Methods. Subcelluar fractions obtained from control (time = 0) and Wnt3a-stimulated cells were analyzed by immunoblotting with anti-PP2A C-subunit antibody. PM (blue line), CY (pink line) and NU (green line) fractions are displayed. Stained protein bands were quantified and the values are presented as "fold of zero time point". Bottom three panels of immunoblots are stained with antibodies against the following subcellular fraction marker proteins: Na⁺-K⁺-ATPase (plasma membrane), GAPDH (cytoplasm) and fibrillarin (nuclei), respectively. The results are shown as mean values ± S.E. from 5 or more independent experiments. Representative blots are displayed, as is the quantitative analysis of cellular content extracted from a compilation of data from the separate experiments (graphs).