Figure 4

Effects of inhibition of PP2A on cellular abundance of Wnt/β-catenin signaling elements. Graphs display the relative cellular abundance of Wnt/β-catenin signaling elements in cells following stimulation with Wnt3a. F9 cells expressing Rfz1 were untreated or treated with Wnt3a in the presence or absence of OA for 0 to 90 min. For OA treatment, F9 cells expressing Rfz1 were treated with OA for 1 hr prior to Wnt3a stimulation. For small t antigen experiments, F9 cells were transiently co-transfected with Rfz1 and small t antigen, and then stimulated with Wnt3a for the indicated times. Cells were collected and lysed. Lysates (60–100 μg protein) were subjected to SDS-PAGE and analyzed by immunoblotting, blots stained with antibodies targeting the signaling molecules indicated. Bands were quantified by densitometry, as described in Experimental Procedure and values are displayed as "fold", with time = 0 set as "1". The results are shown as mean values ± S.E. from 6–8 independent experiments. Representative blots are displayed, as is the quantitative analysis of cellular content extracted from 6–8 separate experiments (graphs).