Figure 6

Interactive effects of curcumin and TRAIL on caspase activation and PARP cleavage. (A and B), PC-3 and LNCaP cells were treated with curcumin (0–40 μM), in the presence or absence of TRAIL, and caspase-3 activity was measured by fluorometric assay as per manufacturer's instructions. (C and D), PC-3 and LNCaP cells were treated with curcumin (0–40 μM), in the presence or absence of TRAIL, and caspase-8 activity was measured by fluorometric assay as per manufacturer's instructions. (E and F), PC-3 and LNCaP cells were treated with curcumin (0, 10 or 20 μM), in the presence or absence of TRAIL (25 nM for PC-3, and 50 nM for LNCaP), and the cleavage of caspase-3, caspase-9, caspase-8 and PARP was measured by the Western blot analysis. β-actin was used as a loading control.