Figure 1

Erk2 binds to human ArhGAP9 at the WW domain. a. Evolutionary conservation of ArhGAP9 and ArhGAP12. Schematic representation of ArhGAP9 and ArhGAP12 domain structures of different species as predicted by SMART domain prediction tool. b. Identification of Erk2 as an interacting protein of the WW domain of human ArhGAP9. Immobilized ArhGAP9-WW-GST precipitated several proteins (bands a-g) from rat brain lysate detected by Colloidal Coomassie Blue. The bands were excised for in-gel reduction, S-alkylation and trypsin hydrolysis. The peptides were identified by mass spectrometry. Bands f and g generated 15 unique peptides covering 40% of the MAP kinase, Erk2 sequence.