Figure 5
From: Estradiol effects on the dopamine transporter – protein levels, subcellular location, and function
GPR30 RNA is present in PC12 cells. The presence of GPR30 ER RNA was detected by RT-PCR using two different primer sets compared to the human breast cancer cell line MCF-7, previously shown to express this receptor. Markers (1 kb DNA Ladder, Invitrogen) were used to determine that the amplimers were of the correct size of 680 and 585 bp, for primer set 1 and 2, respectively.