Skip to main content
Figure 6 | Journal of Molecular Signaling

Figure 6

From: Forkhead box O1 and muscle RING finger 1 protein expression in atrophic and hypertrophic denervated mouse skeletal muscle

Figure 6

FoxO1 protein expression and phosphorylation levels in cytosolic and nuclear fractions of 6-days denervated atrophic anterior tibial muscle. Total FoxO1 protein expression (A) and phosphorylation levels (B) in cytosolic (C) and nuclear (N) fractions of 6-days denervated (Den) atrophic anterior tibial muscle compared to innervated (Inn) controls. Representative images of Western blots are shown together with densitometric quantifications. One innervated cytosolic sample was loaded onto all gels as a reference. All samples were measured relative to this reference. The data were normalized so that the sum of cytosolic and nuclear signals in innervated muscles will give a mean value of 100.0. Mean values ± standard error of the mean. Statistical comparisons were made between cytosolic fractions of denervated versus innervated muscles and between nuclear fractions of denervated versus innervated muscles. **p < 0.01, n = 8 denervated anterior tibial muscles and 8 contralateral innervated control muscles. Each muscle was fractionated into a cytosolic and a nuclear fraction.

Back to article page