SP induces Akt phosphorylation in HeLa cells. (A) Akt phosphorylation in HeLa S3 cells treated with SP (1:50) or control for 0, 5, 10, 20, 40, 60, 120, and 240 min. (B) Akt phosphorylation in HeLa cells treated for 60 min with SP (1:50) or control in the presence/absence of chemical inhibitors/antagonist EGFR kinase [AG-1478;100 nM], PI3 kinase [LY-294002; 25 μM] and EP2 antagonist [AH-6809; 20 μM]. Cell lysate were subjected to immunoblot analysis. Data are represented as mean ± SEM form 3 independent experiments. Paired T-tests were conducted on the untransformed means of the replicates between SP and control and unpaired T-tests performed on SP versus SP and inhibitor after conversion to fold increases. *indicates P < 0.05.