Figure 5
EP2 antagonist and EGFR and PI3 kinase inhibitors, inhibit PGE2 or PGE2 and EGF or EGF mediated induction of IL-1α in HeLa cells. EP2 antagonist, EGFR and PI3 kinase inhibitors inhibit PGE2 (A), PGE2 and EGF (B), and EGF (C) mediated induction of IL-1α in HeLa cells as determined by qPCR. HeLa cells were treated for 4, 8 and 16 hours with EGF [10 ng/mL], or PGE2 [300 nM] or both or vehicle in the absence/presence of antagonist/inhibitors AH6809 [20 μM], AG1478 [100 nM], AH6809 together with AG1478, and LY294002 [25 μM]. Data are represented as mean ± SEM from 5 independent experiments. Paired T-tests were conducted on the untransformed means of the replicates between ligand/ligands treated cells and control and unpaired T-tests performed on ligand/ligands versus ligand/ligands and inhibitor after conversion to fold increases. * and ** indicates significance at P < 0.05 and P < 0.01, respectively.