Figure 4

Induction of IL-1α mRNA in HeLa cells by PGE2 and Butaprost with and without EGF. Induction of IL-1α mRNA in HeLa cells by PGE₂ (A), EGF (B), PGE₂ and EGF (C), butaprost (D) and butaprost and EGF (E) as determined by qPCR. HeLa cells were treated for 4, 8, 16 and 24 hours with PGE₂ [300 nM] or human recombinant EGF [10 ng/mL] or butaprost [5 μM] or butaprost together with EGF or PGE₂ together with EGF or vehicle as control. Data are represented as mean ± SEM from 5 independent experiments. Paired T-tests were conducted on the untransformed means of the replicates between PGE₂ or EGF or butaprost or butaprost and EGF or PGE₂ and EGF treated cells and control. *, ** and *** indicates significance at P < 0.05, P < 0.01, and P < 0.001, respectively.