Induction of IL-1α mRNA in HeLa cells by PGE2 and Butaprost with and without EGF. Induction of IL-1α mRNA in HeLa cells by PGE2
(A), EGF (B), PGE2 and EGF (C), butaprost (D) and butaprost and EGF (E) as determined by qPCR. HeLa cells were treated for 4, 8, 16 and 24 hours with PGE2 [300 nM] or human recombinant EGF [10 ng/mL] or butaprost [5 μM] or butaprost together with EGF or PGE2 together with EGF or vehicle as control. Data are represented as mean ± SEM from 5 independent experiments. Paired T-tests were conducted on the untransformed means of the replicates between PGE2 or EGF or butaprost or butaprost and EGF or PGE2 and EGF treated cells and control. *, ** and *** indicates significance at P < 0.05, P < 0.01, and P < 0.001, respectively.