Expression of IL-1α mRNA and protein in cervical squamous cell carcinoma, adenocarcinoma and normal cervix. A, Relative mRNA expression of IL-1α in (I) cervical squamous cell carcinoma (Ca.1-Ca.7, adenocarcinoma (Ca.11), and normal cervix (N1-N8). (II) Mean relative IL-1α mRNA expression in neoplastic and normal cervical tissue explant as determined by qPCR and shown in (I). B, IL-1α expression by immunohistochemistry (IHC) staining. I, II, III shows IHC, hematoxylin (HE), and diaminobenzidine (DAB) stain of epithelial cells of squamous cell carcinoma, respectively. IV-VI shows IHC, HE, and DAB stain of columnar and glandular epithelium of adenocarcinoma, respectively. VII-IX shows IHC, HE, and DAB stain of normal tissue, respectively. Minimal IL-1α signal was detected in normal cervical tissue (VII and IX). X-XII is control and shows IHC, HE, and DAB stain of normal tissue, respectively. IL-1α staining was abolished in sections incubated with the biotinylated secondary antibody alone (negative control) (X and XII). Scale bar, 50 μm. Data represented as mean ± SEM. **, indicates statistical significance at P < 0.01.