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Figure 7 | Journal of Molecular Signaling

Figure 7

From: Breast cancer cell invasion mediated by Gα12 signaling involves expression of interleukins-6 and −8, and matrix metalloproteinase-2

Figure 7

ChIP assays reveals Gα12QL-dependent increase of NF- κ B occupancy of IL-6, IL-8, and MMP-2 promoters. ChIP assays were performed on chromatin isolated from MDA-MB-231 cells transfected with mock (M) or Gα12QL vector expression vectors. Samples were pre-cleared by a precipitation protocol using control IgG, and then subjected to immunoprecipitation with anti-NF-κB. Semi-quantitative PCR was performed with primers targeted to a consensus NF-κB binding site upstream of the 5′-end of the corresponding genes. The sequences of neighboring regions were used as the “off target” sequence. The quantitative data reflect occupancy and represent mean ± SE of three independent experiments.

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