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Figure 5 | Journal of Molecular Signaling

Figure 5

From: Breast cancer cell invasion mediated by Gα12 signaling involves expression of interleukins-6 and −8, and matrix metalloproteinase-2

Figure 5

Interleukins and MMP-2 are involved in Gα12-mediated invasion of MDA-MB-231 cells. MDA-MB-231 cells were transfected with either control vector (Mock) or Gα12QL vectors as indicated. In addition, for (A) the cells were co-transfected with either scrambled (scRNA) or MMP-2 siRNA; the plasmid vectors also expressed GFP. The green cells were then sorted, and the enriched fractions were subjected to invasion assays. Depletion of MMP-2 with specific siRNA decreases Gα12-stimulated invasion of MDA-MB-231 cells. Data is pooled from three independent experiments, each involving duplicate determinations. Bars represent the mean ± S.E.; *(p < 0.05). (B) Representative western blot showing the over-expression of Gα12QL after doxycycline induction in MDA-MB-231 stable cell line. (C) Addition of IL-6 and IL-8 specific antibodies to the media of MDA-MB-231 cells stably expressing Gα12QL impairs Gα12-stimulated cell invasion. Data is pooled from three independent experiments, each involving triplicate determinations. Bars represent the mean ± S.E.

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