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Figure 4 | Journal of Molecular Signaling

Figure 4

From: Breast cancer cell invasion mediated by Gα12 signaling involves expression of interleukins-6 and −8, and matrix metalloproteinase-2

Figure 4

Validation of increased secretion of IL-6, IL-8 and MMP-2 expression of MDA-MB-231 cells expressing dominant activate Gα12. (A) Expression of IL-6 and IL-8 in total cell lysates of MDA-MB-231 cells transfected with either mock (M) vector or Gα12QL (QL) vector. MDA-MB-231 cells were transfected with the indicated construct and cell lysates prepared 72 h later and subject to immunoblot analysis as described under Methods. (B) MMP-2 levels in conditioned media of MDA-MB-231 cells transfected with either mock (M) vector or Gα12QL (QL) vector. MDA-MB-231 cells were transfected with the indicated construct and media harvested 72 h later and subject to immunoblot analysis as described under Methods. (C) Zymography analysis of gelatinase activity of MMP-2 in conditioned media of MDA-MB-231 cells transfected with either mock (M) vector or Gα12QL (QL) vector. Experimental conditions are as in (B). In the center panel, aliquots of conditioned media from were analyzed by silver-as the loading controls. Data in the lower panel are the mean ± S.E. of quadruplicate determinations from a single experiment that is representative of two independent experiments.

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Journal of Molecular Signaling

ISSN: 1750-2187