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Figure 1 | Journal of Molecular Signaling

Figure 1

From: Breast cancer cell invasion mediated by Gα12 signaling involves expression of interleukins-6 and −8, and matrix metalloproteinase-2

Figure 1

Dominant active Gα12 increases invasiveness of MDA-MB-231 breast cancer cells via paracrine signaling. (A) Experimental scheme of the in vitro invasion assay to test hypothesis that MDA-MB-231 cells transfected with Gα12QL drive the invasion of neighboring untransfected cells. Following transfection with the indicated vectors, cells were sorted and enriched fractions of RFP/mock (M) or GFP/Gα12QL (QL) containing cells (each at ~98% purity), or a 1:1 mixture of these cells, were subjected to the invasion assay followed by FACS analyses. (B) Results from FACS analysis showing invasion of the population of RFP/mock, GFP/Gα12QL and a mixed population of both RFP/mock and GFP/Gα12QL cells. Invaded cells were counted and plotted as a percentage of total cells subjected to the analysis. Values are plotted as the mean ± S.E. The results are from a single experiment that is representative of three independent experiments.

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Journal of Molecular Signaling

ISSN: 1750-2187