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Figure 1 | Journal of Molecular Signaling

Figure 1

From: Identification of endoglin-dependent BMP-2-induced genes in the murine periodontal ligament cell line PDL-L2

Figure 1

Endoglin-dependent BMP-2-induced expression of Id4. (A) The Id4 mRNA levels in PDL-L2 cells that underwent the indicated treatment were quantitatively determined by real-time PCR. The relative mRNA levels of endoglin relative to GAPDH in vehicle-treated PDL-L2 cells without endoglin knockdown were set at 1. The data are expressed as the mean ± SE (n = 3). aSignificant compared to vehicle (Veh)-treated cells, P < 0.05. bSignificant compared to siCont-treated cells exposed to BMP-2, P < 0.05. (B) Effect of BMP-2 and endoglin knockdown on ID4 protein levels in PDL-L2 cells. The lysates of siCont- or siENG-treated PDL-L2 cells exposed to vehicle (Veh) or BMP-2 for 12 h were analysed for ID4 protein levels by Western blotting. The fold increases in the relative band intensities of ID4 normalised to that of β-actin are shown beneath the blots. The data are presented as the mean ± SE (n = 4). *P < 0.05.

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Journal of Molecular Signaling

ISSN: 1750-2187