Figure 5

Hsp70 protein expression in cytosolic and nuclear fractions of 6-days denervated atrophic and 6-days denervated hypertrophic muscle. Hsp70 expression in cytosolic (C) and nuclear (N) fractions of 6-days denervated (Den) atrophic anterior tibial muscle (a) and in 6-days denervated hypertrophic hemidiaphragm muscle (b) compared to innervated (Inn) controls. Representative images of Western blots are shown together with densitometric quantifications. The labeling of the histograms also applies to the lanes in the Western blot images above the histograms. One innervated cytosolic sample was loaded onto all gels as a reference. All samples were measured relative to this reference. The data were normalized so that the sum of cytosolic and nuclear signals in innervated muscles will give a mean value of 100.0. Mean values ± standard error of the mean. Statistical comparisons were made between cytosolic fractions of denervated versus innervated muscles and between nuclear fractions of denervated versus innervated muscles. *p < 0.05, ***p < 0.001, n = 8 denervated anterior tibial muscles and 8 contralateral innervated control muscles (a) and 8 denervated hemidiaphragm muscles and 8 innervated control hemidiaphragms from separate animals (b). Each muscle was fractionated into a cytosolic and a nuclear fraction.