Figure 3
Total and phosphorylated p38, MK2 and Hsp25 in cytosolic and nuclear fractions of 6-days denervated atrophic anterior tibial muscle. Total and phosphorylated p38 (a, b), MK2 (c-e) and Hsp25 (f, g) in cytosolic (C) and nuclear (N) fractions of 6-days denervated (Den) atrophic anterior tibial muscle compared to innervated (Inn) controls. Representative images of Western blots are shown together with densitometric quantifications. The labeling of the histograms also applies to the lanes in the Western blot images above the histograms. All bands shown on the Western blot images were analyzed together. One innervated cytosolic sample was loaded onto all gels as a reference. All samples were measured relative to this reference. The data were normalized so that the sum of cytosolic and nuclear signals in innervated muscles will give a mean value of 100.0. Mean values ± standard error of the mean. Statistical comparisons were made between cytosolic fractions of denervated versus innervated muscles and between nuclear fractions of denervated versus innervated muscles. *p < 0.05, **p < 0.01, ***p < 0.001, n = 8 denervated anterior tibial muscles and 8 contralateral innervated control muscles. Each muscle was fractionated into a cytosolic and a nuclear fraction.