Skip to main content
Figure 4 | Journal of Molecular Signaling

Figure 4

From: Direct RNA sequencing mediated identification of mRNA localized in protrusions of human MDA-MB-231 metastatic breast cancer cells

Figure 4

Characterization of p0071 mRNA and protein localization. (A) DRS and RT-PCR analysis of p0071 mRNA localization in MDA-MB-231 cell protrusions. The expression level of ARPC3 and p0071 mRNA in PF and CF were determined by RT-qPCR and the p0071 mRNA localization ratio normalized to ARPC3 mRNA given the value 1. p0071 mRNA was detected by primers recognizing all p0071 mRNA isoforms (pan-p0071). (B) Protein localization of p0071 in MDA-MB-231 and MCF7 cells. α–Tubulin (left panels) and p0071 (right panels) were visualized by immunofluorescence analysis in MDA-MB-231 cells (upper panels) and MCF7 cells (lower panels). Cells were counterstained with DAPI to visualize nuclei. A representative p0071 staining in a cell protrusion is indicated by arrowhead. Scale bar 20 μm (left panels) and 10 μm (right panels).

Back to article page

Journal of Molecular Signaling

ISSN: 1750-2187