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Figure 1 | Journal of Molecular Signaling

Figure 1

From: EGF regulates tyrosine phosphorylation and membrane-translocation of the scaffold protein Tks5

Figure 1

Tks5 is tyrosine phosphorylated upon EGF treatment of cells. (A) Serum-starved A431 cells were stimulated with EGF (50 ng/ml) for 10 min, and then endogenous Tks5 was immunoprecipitated (IP) with a polyclonal anti-Tks5 antibody. After SDS-PAGE and transfer to nitrocellulose, samples were analysed by anti-phosphotyrosine and anti-Tks5 antibodies. (B) Wild type, V5 epitope-tagged Tks5 was transiently expressed in COS7 cells and then serum-starved cells were stimulated with EGF (50 ng/ml) for 10 min or left untreated. Cell lysates were immunoprecipitated with anti-V5 antibody and the immunoprecipitates were immunoblotted with anti-phosphotyrosine and anti-V5 antibodies. (C) V5 epitope-tagged Tks5 was transiently expressed in COS7 cells and then serum-starved cells were stimulated with EGF (50 ng/ml) as indicated. Cell lysates were immunoprecipitated with anti-V5 antibody and the immunoprecipitates were immunoblotted with anti-phosphotyrosine and anti-V5 antibodies. These results are representative of three experiments.

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