Members of NFAT family and schematic diagram of NFAT5 functional domains. A) Alignment of NFAT1-4 with NFAT5. The DNA-binding domains (Rel homology domain) are aligned. The calcineurin-binding domain present in NFAT1-4 is absent in NFAT5. B) Overall structure of human NFAT5 protein. Sequence and phosphorylation sites in proximity to the AED are indicated. The corresponding amino acid residues are in parenthesis.