Phosphorylated Akt and correlation to PTEN. A) Western blot of primary neuroblastoma tumors showing levels of p-Akt/PKB (S473), p-Akt/PKB (T308), Akt/PKB and PTEN. AG = adrenal gland. B) Levels of p-Akt/PKB (T308), p-Akt/PKB (S473) and PTEN in stage 1–2 (st 1–2) and stage 4 (st 4) neuroblastoma tumors. Relative quantification (RQ) was calculated using GAPDH as endogenous control. C) Correlation plot using Spearman Rho showing that levels of phosphorylated Akt/PKB (T308) are inversely correlated with protein levels of PTEN.