Stage dependent expression of the PI3K Type IA regulatory isoforms. A) Protein levels of the regulatory subunits of Type IA PI3K: p85α/β, p55α, p50α and p55γ were analyzed with western blot using two sets of primary neuroblastoma tumors. Abbreviations: AG, adrenal gland; B) Relative quantification (RQ) of p85α/β, p55α, p50α and p55γ protein levels using Gapdh as endogenous control. Boxplot explanation; upper hinge of the box, 75th percentile; lower hinge of the box, 25th percentile; thick horizontal line within box, median. The whiskers are indicating range, open circles represent outliers while asterisks represent extremes. C) Protein levels of regulatory subunits in neuroblastoma cell lines (to the left), and cell lines of other origins (to the right).