Determinants at the N- and C-termini of Gα 12 required for activation of Rho-mediated signaling

Table 2 Gα ₁₂ cassette mutants corresponding to rgRGS contact points within Gα ₁₃

Gα ₁₃residues in contact with p115-RH	myc-Gα₁₂ ^QLNAAIRS mutant
Val⁹⁸	Q
Asp¹⁰¹, Ala¹⁰²	R
Lys¹⁰⁵, Leu¹⁰⁶	S
Thr¹²⁷, Arg¹²⁸	V
Phe¹⁶⁸	BB
Arg²⁰⁰, Pro²⁰², Lys²⁰⁴	HH
Gln²²⁶	LL
Arg²³⁰, Lys²³¹, Phe²³⁴	MM
Met²⁵⁷	QQ
Arg²⁶⁰	RR
Asn²⁷⁰	SS
Ile²⁷¹, Glu²⁷³, Thr²⁷⁴, Ile²⁷⁵	TT
Asn²⁷⁸, Arg²⁷⁹, Val²⁸⁰	UU
Arg³³⁵	DDD

Gα₁₃-native residues previously identified as providing contact points with the RH domain of p115RhoGEF [20, 21] are indicated in the left column. Cassette mutants (“NAAIRS” substitution) in which the homologous residue(s) within Gα₁₂ have been altered are indicated in the right column. See Figure 2 for Gα₁₂ mutant designations.