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Table 2 12 cassette mutants corresponding to rgRGS contact points within Gα 13

From: Determinants at the N- and C-termini of Gα12 required for activation of Rho-mediated signaling

13residues in contact with p115-RH

myc-Gα12 QLNAAIRS mutant

Val98

Q

Asp101, Ala102

R

Lys105, Leu106

S

Thr127, Arg128

V

Phe168

BB

Arg200, Pro202, Lys204

HH

Gln226

LL

Arg230, Lys231, Phe234

MM

Met257

QQ

Arg260

RR

Asn270

SS

Ile271, Glu273, Thr274, Ile275

TT

Asn278, Arg279, Val280

UU

Arg335

DDD

  1. 13-native residues previously identified as providing contact points with the RH domain of p115RhoGEF [20, 21] are indicated in the left column. Cassette mutants (“NAAIRS” substitution) in which the homologous residue(s) within Gα12 have been altered are indicated in the right column. See Figure 2 for Gα12 mutant designations.