|
70-100%
|
A-D F-H L N O T-V X BB DD-FF II KK XX YY BBB DDD III-KKK
|
|
40-70%
|
H P GG JJ WW ZZ CCC
|
|
20-40%
|
Y AA PP
|
|
10-20%
|
I OO QQ-SS UU VV FFF
|
|
0-10%
|
E J K M Q R S Z HH LL-NN TT AAA EEE GGG HHH
|
|
N/D
|
W CC
|
- Cassette substitution mutants of myc-Gα12
QL (see Figure 2 for alphabetical designations) were expressed in HEK293 cells and subjected to protein interaction assays using a GST-fusion of the RH domain of LARG as described in Methods, and for each mutant a pulldown:load ratio was determined and calculated as a percent (left column) of the same ratio for unmodified myc-Gα12
QL assayed in parallel. Each Gα12 mutant was analyzed in three independent experiments, except for mutants that appeared in the 70-100% category in two independent experiments.
