Figure 6
From: Determinants at the N- and C-termini of Gα12 required for activation of Rho-mediated signaling
Structural position of Gα 12 C-terminal determinants of RhoGEF binding. The structure of N-terminally Gαi1-substituted Gα12 (PDB accession code 1ZCA, [22]) as a GDP•AlF4 ¯ activated complex was analyzed using PyMOL software. The native Gα12 region substituted for the sequence “NAAIRS” in the C-terminal mutants EEE, FFF, and GGG is highlighted in orange, and the sextet substituted in mutant HHH is highlighted in black. The bound GDP molecule is highlighted in blue. Figure was rendered in The PyMOL Molecular Graphics System, Version 1.5.0.1 Schrödinger, LLC.