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Figure 5 | Journal of Molecular Signaling

Figure 5

From: Caspase-3 mediated release of SAC domain containing fragment from Par-4 is necessary for the sphingosine-induced apoptosis in Jurkat cells

Figure 5

Inhibition of Akt dephosphorylation protects from SPH-induced Par-4 cleavage and apoptosis. Panel A , Jurkat cells were pre-treated with 5 nM calyculin A (Cal A) and 30 μM phosphatidic acid (PA) followed by treatment with 8 μM SPH. Cells were lysed and fractionated by SDS-PAGE. Western blot were probed with antibodies specific for phospho-Akt (Ser473), Akt, Par-4 and PARP. Actin was used as loading control. Panel B , Jurkat cells were pre-treated with 5 nM Cal A and 30 μM PA followed by treatment with 8 μM SPH and DNA fragmentation analysis was performed. Panel C, Jurkat cells were pre-treated with 5 nM CalA and 30 μM PA followed by treatment with 8 μM SPH and viability assay was done by using WST assay kit. Data shown are means ± SD (n = 3). *, p < 0.05, and **, p < 0.05 compared with untreated control and SPH treated cells, respectively.

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Journal of Molecular Signaling

ISSN: 1750-2187