Figure 2

Effects of inhibitors on H ₂ O ₂ signal and receptor autophosphorylation in neurons stimulated with 100 nM insulin. (A) Left Y axis: malonate dose–response for receptor autophosphorylation in CGN exposed to insulin (triangles, mean±SEM of 3-11 cultures, *P<0.05 vs. insulin). Right Y axis: malonate effect on Rhodamine 123 fluorescence in CGN (circles, mean±SEM of 3-7cultures, *P<0.05 vs. control). (B) Left Y axis: kinetics of H₂O₂ efflux from CGN exposed to vehicle (mean of 3 cultures), 6 mM malonate (mean of 3 cultures), insulin (mean of 10 cultures), or insulin plus 6 mM malonate (mean of 5 cultures). Right Y axis: rates of H₂O₂ efflux from CGN exposed to insulin (red line) or insulin plus 6 mM malonate (grey line). (C) Left Y axis: receptor autophosphorylation in CGN exposed to vehicle, 6 mM malonate, insulin, or insulin plus 6 mM malonate (mean±SEM of 5-11 cultures. *P<0.05 vs. control. ^# P<0.05 vs. insulin). Right Y axis: areas under curves of H₂O₂ efflux from CGN exposed to vehicle, 6 mM malonate, insulin, or insulin plus 6 mM malonate for 30 s (mean±SEM of 3-10 cultures. *P<0.05 vs. control. ^# P<0.05 vs. insulin). (D) Left Y axis: rotenone dose–response for receptor autophosphorylation in CGN exposed to insulin (triangles, mean±SEM of 3-4 cultures, *P<0.05 vs. insulin). Right Y axis: rotenone effect on Rhodamine 123 fluorescence in CGN (circles, mean±SEM of 3-4 cultures, *P<0.05 vs. control). (E) Left Y axis: FCCP dose–response for receptor autophosphorylation in CGN exposed to insulin (triangles, mean±SEM of 3-5 cultures, *P<0.05 vs. insulin). Right Y axis: FCCP dose-response for Rhodamine 123 fluorescence in CGN (circles, mean±SEM of 4-8 cultures, *P<0.05 vs. control). (F) DPI effect on receptor autophosphorylation in CGN exposed to insulin (mean±SEM of 3 cultures, *P<0.05 vs. insulin).