Figure 2

OSI-906 activates PKA in a TGFβ signaling dependent manner. FET cells pretreated treated with TGFβRI inhibitor ALK5i (400 nM) abrogated OSI-906 (1 μM) mediated PKA activation. FET cells treated with TGFβ (5 ng/ml) and OSI-906 together showed an increase in PKA activation (A). OSI-906 mediated PKA activation is Smad3-dependent as determined by knockdown of Smad3 in FET cells (B). All experiments were repeated three times independently with 3 replicas per repeat for the ANOVA analysis (* P < 0.001).