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Figure 1 | Journal of Molecular Signaling

Figure 1

From: Differential PKA activation and AKAP association determines cell fate in cancer cells

Figure 1

OSI-906 mediates cAMP independent and AKAP dependent PKA activation in colon cancer cells. FET cells treated with OSI-906 (1 μM) activated PKA in a time dependent fashion (A). Forskolin (10 μM) was used as a positive control. PKA inhibitor H89 (15 μM) was used to inhibit PKA activation. Pretreatment with H89 followed by OSI-906 treatment abrogated the PKA activation (B). Knockdown of PKA Catalytic subunit (FETCatKD) abrogated OSI-906 mediated PKA activation. However, FET cells transfected with Scrambled siRNA (FET Scr) cells showed PKA activation following OSI-906 treatment. (C). PKA activation by OSI-906 is cAMP independent as determined via cAMP assay (D). All experiments were repeated three times independently with 3 replicas per repeat for the ANOVA analysis. (* P < 0.001).

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