Figure 5
From: Assembly of Dishevelled 3-based supermolecular complexes via phosphorylation and Axin
Formation of Dvl3-based punctae by wild-type versus mutant forms of Dvl3. Y17D-Dvl3 forms punctae in cells co-expressing wild-type Dvl3 in untreated and Dvl3-deficient F9 cells. Panel A, F9 cells were co-transfected with GFP- and HA-tagged Y17D-Dvl3 and increasing amount of co-expressed untagged wild-type Dvl3 (ratio = 1 : 0.2, or 1 : 2). Post 1 day transfection, cells were stimulated with Wnt3a. Cell images were taken during Wnt stimulation. Results were displayed at 0, 7.5, and 15 min time-points. Panel B, parallel experiments were performed in F9 cells made deficient of endogenous wild-type Dvl3 by siRNA. The images shown are representative of three or more separate experiments.