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Figure 4 | Journal of Molecular Signaling

Figure 4

From: Akt (protein kinase B) isoform phosphorylation and signaling downstream of mTOR (mammalian target of rapamycin) in denervated atrophic and hypertrophic mouse skeletal muscle

Figure 4

Akt and GSK-3β protein and phosphorylation levels in 6-days denervated atrophic anterior tibial muscle. Expression of Akt1, Akt2 and GSK-3β total protein (t-Akt1, a; t-Akt2, c and t-GSK-3β, e) and phosphorylated Akt1 protein (p-Akt1) at S473 (b), phosphorylated Akt2 protein (p-Akt2) at S474 (d) and phosphorylated GSK-3β protein (p-GSK-3β) at S9 (f) in 6-days denervated atrophic anterior tibial (Den) muscle compared to innervated (Inn) controls. Representative Western blots are shown together with densitometric quantifications. One innervated anterior tibial muscle sample was used as a reference sample and was included in all gels. All other samples were measured relative to this reference. The data were normalized to give an average signal of 100.0 in innervated muscles. Mean values ± standard error of the mean. *p < 0.05, **p < 0.01, ***p < 0.001.

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