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Figure 3 | Journal of Molecular Signaling

Figure 3

From: Akt (protein kinase B) isoform phosphorylation and signaling downstream of mTOR (mammalian target of rapamycin) in denervated atrophic and hypertrophic mouse skeletal muscle

Figure 3

4EBP1, p70S6K1 and rpS6 protein and phosphorylation levels in 6-days denervated hypertrophic hemidiaphragm muscle. Expression of 4EBP1, p70S6K1 and rpS6 total protein (t-4EBP1, a; t-p70S6K1, c and t-rpS6, e) and phosphorylated 4EBP1 (p-4EBP1) at S65 (b), phosphorylated p70S6K1 (p-p70S6K1) at T389 (d) and phosphorylated rpS6 (p-rpS6) at S235/236 (f) in 6-days denervated hypertrophic hemidiaphragm muscle (Den) compared to innervated (Inn) controls. Representative Western blots are shown together with densitometric quantifications. One innervated hemidiaphragm muscle sample was used as a reference sample and included in all gels. All other samples were measured relative to this reference. The data were normalized to give an average signal of 100.0 in innervated muscles. Mean values ± standard error of the mean. **p < 0.01, ***p < 0.001.

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Journal of Molecular Signaling

ISSN: 1750-2187