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Figure 3 | Journal of Molecular Signaling

Figure 3

From: Chibby suppresses growth of human SW480 colon adenocarcinoma cells through inhibition of β-catenin signaling

Figure 3

Cby facilitates nuclear export of endogenous β-catenin. (A) SW480 cells stably expressing CbyWT or CbyS20A and control cells were fixed and subjected to immunofluorescence staining of endogenous β-catenin (green). Nuclei were visualized with DAPI. (B) Whole-cell (W), nuclear (N) and cytoplasmic (C) extracts were prepared from the indicated SW480 stable cells, and β-catenin levels were analyzed by western blotting. The relative purity of nuclear and cytoplasmic fractions was evaluated by probing for nucleoporin p62 (NUP62) and GAPDH, respectively.

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Journal of Molecular Signaling

ISSN: 1750-2187