Figure 8

Representative photomicrographs illustrating TUNEL positive cells as an index of apoptosis. HEK-293 cells cotransfected with SSTR2/SSTR3 were treated with SST (1, 50 nM and 1 μM), L-779976 (10 and 100 nM) and L-796778 (25 and 50 nM) for 24 h, and processed for TUNEL staining. SST and receptor-specific agonists increased TUNEL labeling indicating cytotoxic role for SSTR2 and SSTR3. Histogram represents quantitative analysis of apoptotic cells. A total of 600–750 cells were counted for each treatment. Arrows in representative panels indicate apoptotic cells. Scale bar = 10 μm. Mean ± S.D is representative of three independent experiments.