Figure 2

Spatial localization of AKAR2-AKAP5 and AKAR2-AKAP12 in resting cells. Fluorescence images of AKAR2, AKAR2-AKAP5, AKAR2-AKAP12 and were recorded at 473-495 nm for CFP and 527-591 nm for YFP, in HEK 293 cells. The nuclei were stained with Hoechst 33258. Individual panels display the signal from CFP only, YFP only, or Hoechst only. The merged image of all three inputs is provided. Experiments were repeated at least three times with equivalent results.