Figure 10

Time-courses of activation of AKAP biosensor, ERK1,2, and of beta-adrenergic receptor desensitization: differential responses of AKAP5 versus AKAP12. HEK 293 cells were stimulated with 10 μM ISO and sampled at times up to 30 min. HEK (A) or A431 (B) cells were sampled for three read-outs: ERK1,2 activation, AKAR2-AKAP biosensor FRET signaling, and beta-adrenergic receptor-mediated desensitization. In HEK cells, AKAR2-AKAP5 activation, ERK1,2 activation and receptor desensitization are displayed in Panel A. In A431 cells, AKAR2-AKAP12 activation, ERK1,2 activation and receptor desensitization are displayed in Panel B. “0% desensitization” denotes the magnitude of the cyclic AMP response to a second challenge with ISO compared to the first challenge. Desensitization data are plotted from earlier time-courses [47, 48].