Table 2 Characterization of WIPI-1 mutants
WIPI-1 | Puncta | PtdIns(3)P | PtdIns(3,5)P2 | Atg12 |
|---|---|---|---|---|
Mutant | formation | binding | binding | colocalization |
GFP-N23A | + | + | + | + |
GFP-Q24A | + | + | + | + |
GFP-D25A | + | + | + | + |
GFP-E64A | + | + | + | + |
GFP-R107A | + | + | + | + |
GFP-R110A | + | + | + | + |
GFP-R112A | + | - | n/a | + |
GFP-H185A | + | - | n/a | n/a |
GFP-G198A | + | + | + | + |
GFP-S203A | - | - | - | - |
GFP-S205A | - | - | - | - |
GFP-G208A | - | - | - | - |
GFP-T209A | - | - | - | - |
GFP-R212A | - | - | - | - |
GFP-E224A | + | + | + | + |
GFP-F225A | + | + | + | + |
GFP-R226A | - | - | - | - |
GFP-R227A | - | - | - | - |
GFP-RR | - | - | - | - |
GFP-G228A | - | - | - | - |
GFP-S250A | + | + | + | + |
GFP-S251A | - | - | - | - |
GFP-T255A | - | - | - | - |
GFP-H257A | - | - | - | - |
GFP-S335A | + | + | + | + |
GFP-G336A | + | + | + | + |