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Figure 8 | Journal of Molecular Signaling

Figure 8

From: Defining regulatory and phosphoinositide-binding sites in the human WIPI-1 β-propeller responsible for autophagosomal membrane localization downstream of mTORC1 inhibition

Figure 8

GFP-WIPI-1 mutants capable of PtdIns(3)P can also bind to PtdIns(3,5)P 2 . Quantification of phospholipid-protein overlay assays using membrane-immobilized PtdIns(3)P and PtdIns(3,5)P2, and native cell extracts from U2OS cells transiently expressing GFP-tagged wild-type or mutant WIPI-1 variants (n = 2). Total intensities of anti-GFP ECL signals at PtdIns(3)P and PtdIns(3,5)P2 positions were set to 100% for each variant and the very approximate percentage of PtdIns(3)P and PtdIns(3,5)P2 binding calculated.

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Journal of Molecular Signaling

ISSN: 1750-2187