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Figure 6 | Journal of Molecular Signaling

Figure 6

From: Defining regulatory and phosphoinositide-binding sites in the human WIPI-1 β-propeller responsible for autophagosomal membrane localization downstream of mTORC1 inhibition

Figure 6

GFP-WIPI-1 binding to PtdIns(3)P and PtdIns(3,5)P 2 . (A) Western blot analysis of endogenous WIPI-1 protein in G361, HeLa or U2OS cells upon treatments with control medium (CM), 233 nM wortmannin (WM) or 300 nM rapamycin (RM). (B) Transient over-expression of GFP-WIPI-1 in G361 (left panel) or HeLa (right panel) cells followed by protein-phospholipid overlay assays using native cell extracts, adjusted to contain equivalent levels of GFP-WIPI-1 protein. (C) Protein-phospholipid overlay assay using native cell extracts from GFP-WIPI-1 or GFP-RR expressing U2OS cells.

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Journal of Molecular Signaling

ISSN: 1750-2187