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Figure 4 | Journal of Molecular Signaling

Figure 4

From: Defining regulatory and phosphoinositide-binding sites in the human WIPI-1 β-propeller responsible for autophagosomal membrane localization downstream of mTORC1 inhibition

Figure 4

Colocalization of endogenous Atg12 with wild-type GFP-WIPI-1 and puncta-formation competent GFP-WIPI-1 mutants upon rapamycin-mediated induction of autophagy. U2OS cells transiently expressing GFP-WIPI-1 or GFP-tagged mutant WIPI-1 variants were treated for 3 h with 300 nM rapamycin, fixed, stained with anti-Atg12/Alexa 546 antibodies (red) and TOPRO3 (nuclei, blue), and analyzed by confocal laser-scanning microscopy. Merged images are shown. Scale bars 20 μM.

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Journal of Molecular Signaling

ISSN: 1750-2187