Skip to main content
Figure 3 | Journal of Molecular Signaling

Figure 3

From: Defining regulatory and phosphoinositide-binding sites in the human WIPI-1 β-propeller responsible for autophagosomal membrane localization downstream of mTORC1 inhibition

Figure 3

Quantitative puncta-formation analysis of GFP-tagged wild-type or mutant WIPI-1 variants in G361 cells. G361 cells transiently expressing GFP-WIPI-1 or GFP-tagged mutant WIPI-1 variants were treated for 3 h with 300 nM rapamycin (RM), fixed and analyzed by confocal laser-scanning microscopy. Quantifications (Additional file 6: Table S2) are presented as the percentage of puncta-positive cells for wild-type and mutant GFP-WIPI-1 variants (300 cells per condition, n = 3). P-values with regard to GFP-WIPI-1: n.s. ≥ 0.05, * < 0.05, ** < 0.01, *** < 0.001. P-values with regard to the corresponding condition in U2OS cells: # < 0.05, ## < 0.01.

Back to article page

Journal of Molecular Signaling

ISSN: 1750-2187