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Figure 2 | Journal of Molecular Signaling

Figure 2

From: Defining regulatory and phosphoinositide-binding sites in the human WIPI-1 β-propeller responsible for autophagosomal membrane localization downstream of mTORC1 inhibition

Figure 2

Quantitative puncta-formation analysis of GFP-tagged wild-type or mutant WIPI-1 variants in U2OS cells. U2OS cells transiently expressing GFP-WIPI-1 or GFP-tagged mutant WIPI-1 variants were treated for 3 h with control medium (CM), 233 nM wortmannin (WM), 300 nM rapamycin (RM), or rapamycin plus wortmannin (RM/WM), fixed and analyzed by confocal laser-scanning microscopy. Representative images and quantifications from all treatments are provided (Additional file 3: Table S1, Additional file 4: Figure S3, Additional file 5: Figure S4). From this, the quantification of rapamycin-treated cells is presented here as the percentage of puncta-positive cells for wild-type and mutant GFP-WIPI-1 variants (300 cells per condition, n = 3). P-values (reference GFP-WIPI-1): n.s. ≥ 0.05, * < 0.05, *** < 0.001.

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Journal of Molecular Signaling

ISSN: 1750-2187