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Figure 5 | Journal of Molecular Signaling

Figure 5

From: NADPH oxidase mediates the oxygen-glucose deprivation/reperfusion-induced increase in the tyrosine phosphorylation of the N-methyl-D-aspartate receptor NR2A subunit in retinoic acid differentiated SH-SY5Y Cells

Figure 5

OGD/R promotes the interaction between activated Src Family Kinases and PSD-95, which is reduced with NADPH oxidase inhibition differentiated SH-SY5Y cells. (A) Immunoprecipitation of PSD-95 and immunoblotting with phospho-SFK(Tyr416) shows the increase in the active form (phospho-Tyr416) of SFKs bound to PSD-95 with treatment of OGD/R compared to normoxic controls (Norm). Western blot is representative of three independent experiments. (B) Quantification of the density of the phospho-SFK(Tyr416) band after PSD-95 immunoprecipitation. (C) Representative Western blot from immunopreciptation of PSD-95 from three independent experiments illustrates the dampening of activated SFKs bound to PSD-95 with DPI (100 nM) or PP2 (1 μM) treatment during reperfusion following 40 minutes of OGD. (D) Quantification of the density of the phospho-SFK(Tyr416) band after PSD-95 immunoprecipitation. (B) Data represent fold change of OGD/R groups compared to normoxic control (arbitrary units) ± S.E.M from three separate experiments. (D) Data represents fold change of treated groups compared to vehicle treated normoxic control group (arbitrary units) ± S.E.M from three separate experiments. (askteriks * indicates a p <0.05 from normoxic control; ** indicates a p <0.001; ANOVA with post hoc Bonferroni test).

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