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Figure 4 | Journal of Molecular Signaling

Figure 4

From: NADPH oxidase mediates the oxygen-glucose deprivation/reperfusion-induced increase in the tyrosine phosphorylation of the N-methyl-D-aspartate receptor NR2A subunit in retinoic acid differentiated SH-SY5Y Cells

Figure 4

The OGD/R-induced increase in tyrosine phosphorylation of the NMDAR NR2A subunit is attenuated by treatment with the Src Inhibitor PP2. Inhibition of Src Family Kinase activity with the selective inhibitor PP2 rescued the increase in tyrosine phosphorylation of the NMDAR NR2A subunit. (A) Representative Western blot from 3 independent experiments illustrates the dampened tyrosine phosphorylation of NR2A with direct inhibition of Src Family Kinases during reperfusion following OGD with PP2 (1 μM). (B) Quantification of the density of the phospho-Tyr-NR2A band. Data represent fold change of treated groups compared to normoxic vehicle treated group (arbitrary units) ± S.E.M from three separate experiments. (askteriks * indicates a p <0.05 from normoxic control; ANOVA with post hoc Bonferroni test).

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Journal of Molecular Signaling

ISSN: 1750-2187