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Figure 3 | Journal of Molecular Signaling

Figure 3

From: NADPH oxidase mediates the oxygen-glucose deprivation/reperfusion-induced increase in the tyrosine phosphorylation of the N-methyl-D-aspartate receptor NR2A subunit in retinoic acid differentiated SH-SY5Y Cells

Figure 3

Inhibition of NADPH Oxidase activity blocks the OGD/R-induced increase in tyrosine phosphorylation of the NMDAR NR2A. Inhibition of superoxide generation from xanthine oxidase with oxypurinol (1 μM) and mitochondria with FCCP (0.5 μM) failed to prevent the OGD/R-induced increase in tyrosine phosphorylation of the NMDAR NR2A subunit. However, inhibition of NADPH Oxidase with DPI (100 nM) was found to block the increase in tyrosine phosphorylation of the NR2A subunit. Representative Western blot (A) of three independent experiments of phospho-Tyr-NR2A with vehicle (1:1000 DMSO), DPI, oxypurinol, and FCCP. (B) Quantification of the density of the phospho-Tyr-NR2A band. Data represent fold change of OGD/R treated groups compared to normoxic control (arbitrary units) ± S.E.M from three separate experiments. (askteriks * indicates a p <0.05 from normoxic control; ANOVA with post hoc Bonferroni test).

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