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Figure 2 | Journal of Molecular Signaling

Figure 2

From: NADPH oxidase mediates the oxygen-glucose deprivation/reperfusion-induced increase in the tyrosine phosphorylation of the N-methyl-D-aspartate receptor NR2A subunit in retinoic acid differentiated SH-SY5Y Cells

Figure 2

OGD/R promotes the increase in tyrosine phosphorylation of the NMDAR NR2A subunit in differentiated SH-SY5Y cells. Immunoprecipitation and immunoblotting of lysates prepared from differentiated SH-SY5Y cells subjected to OGD/R reveals a significant increase in tyrosine phosphorylation of the NR2A subunit. (A) Western blot shown is representative of three independent experiments. (B) Quantification of the relative densitometry of the phospho-Tyr-NR2A immunoreactive band. Data represent fold change of OGD/R groups compared to normoxic control group (arbitrary units) ± S.E.M from three separate experiments. (askteriks * indicates a p <0.01 from normoxic control; ANOVA with post hoc Bonferroni test).

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Journal of Molecular Signaling

ISSN: 1750-2187