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Figure 8 | Journal of Molecular Signaling

Figure 8

From: Heterodimerization of β2 adrenergic receptor and somatostatin receptor 5: Implications in modulation of signaling pathway

Figure 8

Receptor mediated changes in ERK1/2 and p38 phosphorylation. Mono-and cotransfected HEK-293 cells were treated with SST (1 μM), L-817818 (10 nM) and formoterol (1 μM) alone or in combination at 37°C for 10 and 30 min (Panels A and B). In monotransfected cells, increased expression of pERK1/2 was observed with more significant changes in SSTR5 expressing cells. Similar pattern was also seen following 30 min incubation albeit to the lesser degree. In comparison, cotransfected cells displayed discernable changes at 10 or 30 min treatments (Panels A and B). Densitometric analysis of western blot is shown for ERK1/2 (Panels C and D). Data analysis was done by using ANOVA and post hoc Dunnett's to compare against basal level (*, p < 0.01). As shown in panels E and F, no significant expression of phosphorylated p38 was observed in mono-and/or cotransfected HEK-293 cells with or without treatment. Data are representative of three independent experiments.

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Journal of Molecular Signaling

ISSN: 1750-2187