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Figure 6 | Journal of Molecular Signaling

Figure 6

From: Heterodimerization of β2 adrenergic receptor and somatostatin receptor 5: Implications in modulation of signaling pathway

Figure 6

Specificity of G protein coupling. Mono-and cotransfected cells were treated with Gs inhibitors GPAP (5 μM) and Melittin (1 μM) for 2 h; 100 ng/ml PTX (Gi inhibitor) for 16-18 h in DMEM at 37°C and processed for cAMP estimation. GPAP and Melittin resulted in complete loss of formoterol induced cAMP levels, whereas PTX had no significant effect on increased cAMP levels upon formoterol treatment in mono and/or cotransfected cells (Panels A and B). In contrast regulation of cAMP levels by SSTR5 were completely abrogated upon PTX treatment in mono or cotransfected cells. Mean ± S.E. are representative of three independent experiments performed in triplicate. Data analysis was done by using ANOVA and post hoc Dunnett's to compare against basal level (*, p < 0.05; **, p < 0.01).

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Journal of Molecular Signaling

ISSN: 1750-2187