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Figure 5 | Journal of Molecular Signaling

Figure 5

From: Heterodimerization of β2 adrenergic receptor and somatostatin receptor 5: Implications in modulation of signaling pathway

Figure 5

Inactivation of β 2 AR enhanced hSSTR5 coupling to adenylyl cyclase. Mono-and/or cotransfected HEK-293 cells were processed as described in materials and methods. In basal condition, cAMP level was relatively higher in cells expressing β2AR (Panel A) in comparison to hSSTR5 expressing cells (Panel A). However, both the cell lines displayed increased cAMP in the presence of FSK. As shown, SSTR5 transfected cells exhibited the inhibition of cAMP in presence of SST and SSTR5 specific agonist. In cotransfected cells, basal level of cAMP was comparable to hSSTR5 expressing cells (Panel B). Note the significant decrease in cAMP with SST in presence of β2AR antagonist (Panel C). Mean ± S.E. are representative of three independent experiments performed in triplicate. Data analysis was done by using ANOVA and post hoc Dunnett's to compare against basal level (*, p < 0.01).

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Journal of Molecular Signaling

ISSN: 1750-2187