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Figure 1 | Journal of Molecular Signaling

Figure 1

From: Heterodimerization of β2 adrenergic receptor and somatostatin receptor 5: Implications in modulation of signaling pathway

Figure 1

Heterodimerization of hSSTR5 and β 2 AR in cotransfected HEK-293 cells. Mono-and/or cotransfected cells were treated as indicated for 30 min at 37°C. A single band at the expected size of ~110 kDa an indicative of β2AR/hSSTR5 heterodimers was observed in β2AR immunoprecipitate (arrow panel A). Same membrane was reprobed for β2AR expression using anti-cMyc specific antibody for the specificity of heterodimers (arrow panel B). A band at the expected size of ~50 kDa was also seen for β2AR monomers. For specificity, monotransfected cells expressing SSTR5 or β2AR were used. No bands at the expected molecular weights were observed in either control or treated condition indicating the specificity of heterodimerization (Panels C and D). Data are representative of three independent experiments.

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